现代农业科技Issue(22)：58-61,64,5.
无核荔枝MYB转录因子基因克隆及进化分析
Cloning and Phylogeny Analysis of MYB Transcription Factor from Seedless Litchi
摘要
Abstract
To obtain the full length of MY B gene and lay the foundation its function research that was differentially expressed in ovule degeneration of seedless litchi. The EST encoding MY B was obtained from young abortive ovule of seedless litchi via SSH(suppression subtractive hybridization),high quality total RNA was extracted and obtained a 1 177 bp full length cDNA sequence by SMAT-RACE method,bioinformatics analysis showed that it included an 879 bp open reading frame,encoded a deduced protein with 292 amino acids,and contained 2 SANT domains,phylogenetic tree showed it got the closest relatives with Glycine max. The results showed that it's a new member of the MY B family.关键词
MYB/无核荔枝/基因克隆/系统进化树Key words
MY B/seedless litchi/gene cloning/phylogenic tree分类
生物科学引用本文复制引用
刘兴地,李明芳,郑楷,郑学勤..无核荔枝MYB转录因子基因克隆及进化分析[J].现代农业科技,2013,(22):58-61,64,5.基金项目
中国热带农业科学院中央级公益性科研院所基本科研业务专项（ITBB120202）。 （）
