现代农业科技Issue(22)：139-141,3.
山荆子CBF转录因子的克隆、序列分析及植物表达载体的构建
Cloning,Sequence Analysis and Plant Expression Vector Construction of CBF Transcription Factor from Malus baccata
摘要
Abstract
The CBF transcription factors were cloned by RT-PCR technique from the plants of Malus baccata.The full-length of open reading frame (ORF) was 756 bp,encoding 252 amino acids;The amino acids sequence owned the characteristics of CBF protein,which contained an AP2/EREB DNA binding domain and two special short amino acids sequences;The amino acids sequences of MbCBF had highly homologous with CBF genes of other plants included in Genebank,reached 98%with Malus domestica,and 67%with Prunus mume respectively;Then we connected the gene into the plant expression vector PBI121,which laid the foundation for the utilization of CBF genes to improve plant stress tolerance.关键词
山荆子/CBF转录因子/克隆/序列分析/表达载体Key words
Malus baccata/CBF transcription factor/clone/sequence analysis/plant expression vector分类
生物科学引用本文复制引用
刘晓丹,徐亚维,叶飞,于晓明..山荆子CBF转录因子的克隆、序列分析及植物表达载体的构建[J].现代农业科技,2013,(22):139-141,3.基金项目
吉林农业科技学院青年教师科研基金资助项目（2012126）。 （）
