现代农业科技Issue(23)：21-24,4.
芝麻高度不育材料基因组DNA提取及RAPD反应体系的建立
Extraction of Genomic DNA and Optimization of the RAPD Reaction System of Sesame Highly Sterile Lines
摘要
Abstract
The genomic DNA extraction and the RAPD-PCR reaction conditions were optimized when using genomes from sesame leaves of temporary maintainer line WB51220,AB Line infertile plant 0176A,fertile plant 0176B,and Wanzhi No.1 as templates. The results indicated that the genomic DNAs extracted from sesame leaves by improved CTAB DNA extraction method satisfied the requirement of genetic diversity analysis. The best reaction condition for RAPD analysis was as follows:Taq DNA polymerase 1.5 U,dNTP 0.25 mmoL/L,Mg2+2.0 mmoL/L,and random primer 0.75μmoL/L in a total volume of 20μL. Electrophoresis results indicated that bands amplified from the genomic DNAs of four varieties had four common bands in length of 1900,1800,1000,and 900 bp,respectively. The differences were that new bands in length of 2000 bp and 450 bp were amplified from AB line infertile plant 0176A when compared with temporary maintainer line WB51220 and fertile plant 0176B,respectively. And two new bands in length of 750 bp and 1200 bp were amplified from fertile plant 0176B when compared with Wanzhi NO.1.关键词
芝麻/保持系/不育株/RAPD反应体系/建立Key words
sesame/maintainer line/infertile plant/RAPD reaction system/optimization分类
农业科技引用本文复制引用
赵莉,孔小卫,汪强,林勇翔,田东丰..芝麻高度不育材料基因组DNA提取及RAPD反应体系的建立[J].现代农业科技,2013,(23):21-24,4.基金项目
安徽省院长青年创新基金（13B0220）；国家芝麻产业技术体系南方栽培与土肥岗位（CARS-15-1-09）。 （）
