现代农业科技Issue(23):270-271,274,3.
Marc-145细胞传代培养条件优化
Optimization of Subculture Conditions for Marc-145 Cells
赵潇 1秦钢1
作者信息
- 1. 浙江经贸职业技术学院,浙江杭州 310018
- 折叠
摘要
Abstract
Using single test,the effect of recovery temperature serum concentration,growth solution pH value,incubation temperature,concentration of digestive fluid and passage rate on the subculture for Marc-145 cells was studied.The results showed that the best subculture conditions for Marc-145 cells was when the recovery temperature was 37℃,the serum concentration was 10%,growth solution pH value was 7.2,incubation temperature was 37℃,concentration of digestive fluid was 0.25%,and passage rate was 1∶3.关键词
Marc-145细胞/传代培养/单因素试验/条件优化Key words
Marc-145 cells/subculture/single test/condition optimization分类
农业科技引用本文复制引用
赵潇,秦钢..Marc-145细胞传代培养条件优化[J].现代农业科技,2013,(23):270-271,274,3.