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Marc-145细胞传代培养条件优化

赵潇 秦钢

现代农业科技Issue(23):270-271,274,3.
现代农业科技Issue(23):270-271,274,3.

Marc-145细胞传代培养条件优化

Optimization of Subculture Conditions for Marc-145 Cells

赵潇 1秦钢1

作者信息

  • 1. 浙江经贸职业技术学院,浙江杭州 310018
  • 折叠

摘要

Abstract

Using single test,the effect of recovery temperature serum concentration,growth solution pH value,incubation temperature,concentration of digestive fluid and passage rate on the subculture for Marc-145 cells was studied.The results showed that the best subculture conditions for Marc-145 cells was when the recovery temperature was 37℃,the serum concentration was 10%,growth solution pH value was 7.2,incubation temperature was 37℃,concentration of digestive fluid was 0.25%,and passage rate was 1∶3.

关键词

Marc-145细胞/传代培养/单因素试验/条件优化

Key words

Marc-145 cells/subculture/single test/condition optimization

分类

农业科技

引用本文复制引用

赵潇,秦钢..Marc-145细胞传代培养条件优化[J].现代农业科技,2013,(23):270-271,274,3.

现代农业科技

1007-5739

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