现代农业科技Issue(2)：175-175,183,2.
外植体不同长度及接种方式对马尾松愈伤组织诱导的影响
Effect of Explant Length and Inoculation Method on Callus Induction of Pinus massoniana Lamb
曾庆玲 1伊书亮 2魏静1
作者信息
- 1. 河南省信阳市浉河区林业局，河南信阳 464000
- 2. 河南信阳毛尖集团生产管理分公司
- 折叠
摘要
Abstract
To study the effect of explant length and inoculation method on callus induction of Pinus massoniana Lamb..Explants of Pinus massoniana Lamb. were divided into different groups according to explants length and inculcation method ,the differences between the groups were compared. The results showed that when inoculated ,the best explants length were 0.1~0.2 cm. A large quantity of callus cells initiated on the surface of cutting areas,the entire explants were completely differentiated into calluses. The highest callus induction rate was 84.00%with seldom browning and the lowest contamination rate. The best explant inoculating method was inoculated vertically. In this case ,the callus formed earlier with a large number of callus cell population. It also had a lower browning and contamination level.关键词
马尾松/外植体长度/接种方式/愈伤组织/诱导Key words
Pinus massoniana Lamb./explant length/inoculation methods/callus/induce分类
农业科技引用本文复制引用
曾庆玲,伊书亮,魏静..外植体不同长度及接种方式对马尾松愈伤组织诱导的影响[J].现代农业科技,2014,(2):175-175,183,2.
