现代农业科技Issue(5)：296-297,300,3.
大肠杆菌MetJ的基因修饰对其蛋氨酸产量的影响
Effect of Gene Modification in Escherichia coli MetJ on Its L-met Production
闫海洋 1林松毅 1欧阳红生2
作者信息
- 1. 吉林大学军需科技学院，吉林长春 130062
- 2. 吉林大学动物科技学院
- 折叠
摘要
Abstract
Gene mutation was carried out using N-methyl-N′-nitro-N-nitrosoguanidine. L-met-analogue-resistant mutants were derived from Escherichia coli K-12. The mutants produced 1 020 mg/L L-met in the fermentation test. It was confirmed that two key enzymes ,cystathionine γ-synthase and cystathionineβ-lyase were not repressed by excess of L-met during fermentation. MetJ protein was cloned and sequenced by the PCR. It was observed that serine at position 54 was replaced by asparaginate ,which enhanced L-met production because of the depression of L-met biosynthetic enzymes.关键词
大肠杆菌/蛋氨酸阻遏蛋白/反馈阻遏/基因修饰/L-蛋氨酸产量Key words
Escherichia coli/MetJ/feedback repression/gene modification/L-met production分类
生物科学引用本文复制引用
闫海洋,林松毅,欧阳红生..大肠杆菌MetJ的基因修饰对其蛋氨酸产量的影响[J].现代农业科技,2014,(5):296-297,300,3.
