安徽医科大学学报Issue(11)：1292-1296,5.
秀丽线虫野生株系N2缺氧的荧光定量蛋白质组学研究
Quantitative proteomic (2D-DIGE) studies of wild type Caenorhabditis elegans strain N2 after hypoxia
摘要
Abstract
Objective To investigate the different proteins in wild type Caenorhabditis elegans(C. elegans) N2 af-ter hypoxia and lay the foundation for studying the response mechanism of C. elegans during hypoxia. MethodsThe worms of N2 were cultured synchronously to L4 stage and treated 10 h hypoxia (0. 2% oxygen). Then the total proteins were extracted and two-dimensional difference gel electrophoresis (2D-DIGE) was performed and the se-lected protein spots were then identified by MALDI/TOF/TOF tandem mass spectrometry and search the database. Two mutant strains (prdx-2, prdx-3) were incubated for 4, 6, 8, 10 and 12 h in sealed hypoxia chamber (0. 2%oxygen). The survival rate was calculated. Results Five significantly different sports were finally identified by MS (ratio>2. 0,P<0.05). Two proteins were significantly upregulated (PRDX-2, PRDX-3), and three were down-regulated upon hypoxic stress(RPL-7,H28O16. 1,VHA-12). The mutant strain of (prdx-2, prdx-3) also showed a hypoxia-sensitive phenotype in subsequent analyses, confirming the proteomic findings and showed that PRDX-2, PRDX-3 play an important role in anti-hypoxia. Conclusion Proteomic research can offer the valuable reference data to study the hypoxia in C. elegans. Changed proteins found after 10 h hypoxia are related to the energy, trans-lation and anti-oxidant, which may lay the foundation of understanding the hypoxia responsive mechanism.关键词
秀丽线虫/野生株系(N2)/荧光定量蛋白质组学/过氧还蛋白Key words
C. elegans/wild type strain (N2)/DIGE/PRDX分类
生物科学引用本文复制引用
李华玲,孔玲,秦艳,刘丹丹,夏靖,胡竹林,陈文飞..秀丽线虫野生株系N2缺氧的荧光定量蛋白质组学研究[J].安徽医科大学学报,2013,(11):1292-1296,5.基金项目
国家自然科学基金(编号:81100862) （编号:81100862）
