安徽医科大学学报Issue(11)：1312-1315,4.
非酒精性脂肪肝病大鼠肝脏PGC-1αmRNA表达的变化
Over-expression of PGC-1αgenes in the liver of rats with induced by high-fat diet nonalcoholic fatty liver disease
摘要
Abstract
Objective To investigate the expression of mRNA of peroxisome proliferators-activated receptor-γcoac-tivator-1α (PGC-1α) in the livers of rats with developing nonalcoholic fatty liver disease (NAFLD) and explore the effects of PGC-1α in pathogenesis on the model of rats with NAFLD. Methods Forty-two male SD rats were ran-domly divided into 2 groups:high-fat group and control group. The rats in high-fat group were fed with high-fat di-et;rats in control group were fed with standard diet. At the end of 2th, 4th, 8th week, the rats were sacrificed. The levels of FBS, FINS in serum were detected, and meanwhile the fasting insulin resistance index (FIRI) was calculated. The expression of PGC-1α mRNA was detected with RT-PCR. Results The expression level of PGC-1α mRNA in livers of rats in high-fat group was significantly higher than that in control group in the 4th and 8th week (P<0.01) and increased significantly with the time (P<0.05,P<0.01); the level of PGC-1α mRNA in high-fat group in the 8th week had positive correlation with FBS, FINS, and FIRI (r=0.618, 0.853, 0.776, P<0.01). Conclusion The expression of PGC-1αmRNA is up-regulated in liver of NAFLD rats, and closely relat-ed to IR.关键词
非酒精性脂肪肝/PGC-1α/胰岛素抵抗/高脂膳食/动态变化Key words
nonalcoholic fatty liver/PGC-1α/insulin resistant/high-fat diet/dynamic change分类
医药卫生引用本文复制引用
蒋建华,管石侠,张宝,唐芸,程靖,侯丽丽,叶良平,徐辉..非酒精性脂肪肝病大鼠肝脏PGC-1αmRNA表达的变化[J].安徽医科大学学报,2013,(11):1312-1315,4.基金项目
安徽省自然科学基金(编号:11040606M200) （编号:11040606M200）
