安徽医科大学学报Issue(11)：1320-1323,4.
TNF-α对哮喘小鼠肺泡灌洗液中白三烯含量的影响
Effect of TNF-αon expressions of leukotrienes in bronchoalveolar lavage fluid in asthma mice model
摘要
Abstract
Objective To investigate the expression of leukotriene D4 and leukotriene E4 in the bronchoalveolar lavage fluid(BALF) in asthma mice model and to explore the role of tumor necrosis factor α(TNF-α) played in asthma pathogenesis. Methods Forty-eight BALB/c mice were randomly assigned into normal control(A group), asthma(B group), inhaled low dose TNF-α(C1 group),inhaled high dose TNF-α(C2 group),injected low dose TNF-α(D1 group)and injected high dose TNF-α groups(D2 group)(n=8 each). The mice were sensitized with ovalbumin to establish the asthmatic model. The histological changes were evaluated by hematoxylin and eosin stai-ning, BALF were collected, the total cell and the cell differentials were counted, and the levels of leukotriene D4 and leukotriene E4 were measured by enzyme-linked immunosorbent assay. SPSS 17.0 software was used to analyze the data. Results ① Typical inflammatory changes were shown in asthma group and TNF-α intervention groups. The total cells, EOS amounts of TNF-αintervention group were significantly higher than those of asthma group. ②The expression of LTD4 and LTE4 in BALF:B group,C group and D group were significantly higher than that in A group(P<0.01), C group and D group were significantly higher than B group(P<0.05), C2 group were signifi-cantly higher than C1 group(P<0.05), D2 group were significantly higher than D1 group(P<0.05), but no sta-tistical significance were obtained between C1 group and D1 group, C2 group and D2 group. Conclusion TNF-αcan significantly increase the expression of LTD4 and LTE4 in BALF in asthmatic mice, promote asthma attack and deteriorate airway inflammation. It may be one of the mechanism of TNF-α involved in asthma.关键词
支气管哮喘/肿瘤坏死因子-α/支气管肺泡灌洗液/白三烯Key words
asthma/tumor necrosis factor α/bronchoalveolar lavage fluid/leukotrienes分类
医药卫生引用本文复制引用
张世杰,宋晓丹,马慧敏,朱春江,欧维琳,莫碧文..TNF-α对哮喘小鼠肺泡灌洗液中白三烯含量的影响[J].安徽医科大学学报,2013,(11):1320-1323,4.基金项目
国家自然科学基金(编号:81260009) （编号:81260009）
广西自然科学基金(编号:桂科0728228) （编号:桂科0728228）
广西卫生厅重点课题资助项目(编号:重2010048) （编号:重2010048）
