安徽医科大学学报Issue(11)：1387-1390,4.
实时定量PCR检测Kif2a mRNA在男性精液中的表达
Detection of Kif2a mRNA in male ejaculate by real-time fluorescence quantitative RT-PCR
摘要
Abstract
Objective To detect the expression of kinesin heavy chain member 2A (Kif2a) in human ejaculate and discuss the significance of Kif2a in ejaculate of infertile man. Methods Human ejaculate samples were obtained from 72 subjects, sperm RNA was extracted from ejaculate cells by using TRIzol reagent after liquefaction and cen-trifugation, and the RNA was used for reverse transcription into cDNA. The amount of Kif2a mRNA was quantified by utilizing real-time quantitative PCR (RT-PCR);Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) was se-lected as housekeeping gene. The subjects were grouped as abnormal and normal based on the clinical diagnosis of the ejaculate. Results The results of RT-PCR showed that the Kif2a mRNA is present in human ejaculates. Con-junction with clinical data of sperm vitality, concentration and total motility (progressive+nonprogressive), the ex-pression of Kif2a mRNA in abnormal groups was lower than that in normal groups, with the difference between them being statistically significant(P<0.05). Conclusion According to the distinct expression of Kif2a mRNA in ab-normal and normal ejaculate, whether the difference has potential impact on spermatogenesis and fertilization should be studied further.关键词
不育/精液RNA/实时定量PCR/Kif2aKey words
infertile/sperm RNA/real-time quantitative PCR/Kif2a分类
医药卫生引用本文复制引用
周浩,董云华,孙杨,李红玲,沈涛,严新民..实时定量PCR检测Kif2a mRNA在男性精液中的表达[J].安徽医科大学学报,2013,(11):1387-1390,4.基金项目
云南省应用基础研究计划项目内设研究机构科研项目(编号:2009NS006) （编号:2009NS006）
人力资源和社会保障部资助项目(编号:[2009]416) （编号:[2009]416）
