安徽医科大学学报Issue(12)：1441-1444,4.
Fas配体对椎间盘细胞经典凋亡通路中关键因子的影响
Effect of Fas ligand on the key factors in apoptotic pathway of intervertebral disc cells
摘要
Abstract
Objective To investigate the apoptotic effect and identify the apoptotic pathway of Fas ligand (FasL) on rat intervertebral disc cells in vitro. Methods ①Rat nucleus pulposus cells were cocultured with different dose of recombinant rat FasL (0, 5, 10, 20, 50) in 1% FBS medium for 24 h, then the expression levels of Fas, Caspase-8, Caspase-9, Caspase-3 and Bid mRNA were detected by RT-PCR, the enzyme activity of Caspase-8, Caspase-9, Caspase-3 was evaluated with enzyme activity detection Kit by enzyme appearance, membrane potential change of mitochondria was determined using fluorescence microscope. Results Nucleus pulposus cells with differ-ent concentrations FasL training after 24 h, cell apoptosis was all observed by Hoechst 33258 dyeing. The levels of Caspase-8, Caspase-3 and Bid mRNA were detected by RT-PCR, the enzymatic activity of Caspase-8 and Caspase-3 rised respectively with the dose of recombinant rat FasL. The mRNA expression level and enzymatic activity of Caspase-9, and membrane potential change of mitochondria were increased by 50 mg/ml FasL. Conclusion Mem-brane apoptotic pathway is the main road of rat NP cells' apoptosis induced by FasL. And mitochondrium apoptotic pathway can be activated by a higher dose of FasL.关键词
椎间盘细胞/凋亡/凋亡途径/Fas 配体Key words
intervertebral disc cells/apoptosis/apoptotic pathway/Fas ligand分类
医药卫生引用本文复制引用
韩敦富,解立俊,吴海燕,胡法梅,尹荷珊..Fas配体对椎间盘细胞经典凋亡通路中关键因子的影响[J].安徽医科大学学报,2013,(12):1441-1444,4.基金项目
山东省医药卫生科技发展计划项目(编号:2011WSA03016) （编号:2011WSA03016）
