安徽医科大学学报Issue(1)：9-12,13,5.
应用免疫磁珠分离法纯化弓形虫速殖子的研究
Immunomagnetic separation for purification of Toxoplasma gondii tachyzoites
摘要
Abstract
Objective To obtain the highly purified tachizoites without contamination of host cells and loss of para-site viability for the diverse purposes of Toxoplasma and toxoplasmosis approach. Methods The peritoneal fluids containing both parasites and redundant host cells were harvested from the mouse, that had been peritoneally inocu-lated with Toxoplasma gondii( T. gondii) Wh3 strain ( genotype China 1 ) . T. gondii soluble antigen was prepared and used to immunize rabbits to obtain polyclonal rabbit anti-Toxoplasma antibodies. The mouse peritoneal exudate containing parasites was subjected to the antibody-coated immunomagnetic beads. The purity, recovery, viability and virulence to mice were tested. Results Immunomagnetic separation technique gave rise to as high as 98.2%of the tachyzoites purity; the removal rate of host cells reached to 96% and the recovery rate of tachyzoites was 73.5%. The viability of tachyzoites was 95 . 6% when detected by the MTS kit of cell proliferation and toxicity de-tection, and no attenuation of viability and virulence to mice was noted. Conclusion A high purity of T. gondii techyzoites are achieved by immunomagnetic technique and no negative impacts of the novel method on the parastes viability and virulence are found, suggesting that the immunomagnetic separation, which is fast and simple to car-ried out, might be a useful in the immune and molecular approach of Toxoplasma and toxoplasmosis.关键词
弓形虫/速殖子/纯化/免疫磁珠分离Key words
Toxoplasma gondii/tachyzoites/purification/immunomagnetic separation分类
医药卫生引用本文复制引用
楼研,沈继龙,程维晟,刘芳,陈鹤,罗庆礼,王林..应用免疫磁珠分离法纯化弓形虫速殖子的研究[J].安徽医科大学学报,2014,(1):9-12,13,5.基金项目
国家重点基础研究发展计划资助(973计划)(编号:2010CB530001) （973计划）
