安徽医科大学学报Issue(2)：164-167,4.
免疫金纳米笼对人乳腺癌细胞靶向光热治疗作用
Immuno gold nanocages on human breast cancer cells targeted photothermal therapy
摘要
Abstract
Objective Based on the solar-thermal conversion characteristics of the gold nanocages, we investigated the photothermal effect and ceel toxicity of immune gold nanocage in a human breast cancer cell MCF-7 . Methods Gold nanocages were obtained through the replacement reaction between silver ( Ag ) cubic nanocrystalline and chloroauric acid( HAuCl4 ) . Then the nanoparticles were modified with anti-CK19 antibody to have gold biological targeting characteristics. In experimental group, gold nanocage-antibody probe was applied to the cell, and then treated with the power density 1. 5 W/cm2 , the wavelength of 808 nm laser for 30 min; in control group, gold nanocage-antibody probe was applied to the cell, but no light treatment, or cells were treated with light only. At the end of each treatment, MCF-7 cells were collected for fluorescence termination agent cell staining and flow cytome-try assay for the photothermal effect. The cytotoxicity was examined by colorimetric method of methyl thiazolyl tet-razolium( MTT) . Results The temperature increased significantly to 48 ℃ in control group, but not in control group ( remained 35 ℃) . Cells in experimental group showed significant apoptosis in fluorescent termination agent cell staining and flow cytometry assay, while the cells in control group showed no apoptosis(P<0.05). MMT ex-periments showed that cytotoxicity of this method was small(P<0. 05). Conclusion Immuno gold nanocages have a specific targeting killing effect on MCF-7 cell, and be used for photothermal therapy.关键词
光热治疗/金纳米笼/乳腺肿瘤/治疗Key words
photothermal therapy/gold nanocages/breast cancer/treatment分类
医药卫生引用本文复制引用
田开亮,朱立新,许小亮,尹乃强,黄乾鹏,赵红川,耿小平..免疫金纳米笼对人乳腺癌细胞靶向光热治疗作用[J].安徽医科大学学报,2014,(2):164-167,4.基金项目
国家自然科学基金(编号:51272246、81172082) （编号:51272246、81172082）
