安徽医科大学学报Issue(2)：186-189,190,5.
MAPK信号通路参与褪黑素对肺腺癌A549细胞的增殖抑制作用
Melatonin inhibits proliferation of human lung adenocarcinoma A549 cell line possibly through MAPK signal pathway
摘要
Abstract
Objective To explore the effect of melatonin on the proliferation of human lung adenocarcinoma cells and its mechanism. Methods Regard lung adenocarcinoma cancer cell strains as the research object. The A549 cells were treated with different concentration of melatonin ( Mel) . The proliferation of A549 cells was observed by MTT assay. The change of cell morphology was observed by light microscope. The expression of MAPK related pro-tein and OPN was analyzed by Western blot. Results Mel could inhibit the proliferation of A549 cells in a dose-dependent and time-dependent manner. Compared with control group, A549 cells in the group treated with Mel be-came lesser, more irregular, bigger intercellular gap, non-overlapping and monolayer. The Western blot analysis il-lustrated that 2. 0mmol/L Mel could significantly decreased the expression of phosphorylation of JNK, p38 and OPN in A549 cells ( P<0 . 05 ) , and the expression of phosphorylation of ERK was increased ( P<0 . 05 ) , compared with control group. Conclusion Mel can inhibit the proliferation of A549 cells in a dose-dependent and time-de-pendent manner, which may be related to decrease the expression of OPN by regulating the phosphorylation level of MAPK signal pathway.关键词
褪黑素/人肺腺癌A549细胞/细胞增殖/信号转导/骨桥蛋白Key words
melatonin/human lung adenocarcinoma A549 cell/cell proliferation/signal transduction/osteopon-tin分类
医药卫生引用本文复制引用
周巧云,桂淑玉,周青,陈飞虎,汪渊..MAPK信号通路参与褪黑素对肺腺癌A549细胞的增殖抑制作用[J].安徽医科大学学报,2014,(2):186-189,190,5.基金项目
国家自然科学基金(编号:81272399) （编号:81272399）
安徽省自然科学基金(编号:90413116) （编号:90413116）
