安徽医科大学学报Issue(2)：194-197,4.
硒-甲基硒代半胱氨酸对MCF-7细胞抗氧化及Survivin基因表达的影响
Effect of Se-methylselenoeystein on antioxidation and the gene expression of Survivin in MCF-7 cells
摘要
Abstract
Objective To investigate the effect of Se-methylselenoeystein( MSC) on the proliferation and apoptosis in breast cancer cell line MCF-7 . Methods The human breast cancer MCF-7 cells were treated with different con-centrations MSC:12.5, 25, 50, 100, 200μmol/L. The cell viability was examined by methyl thiazolyl tetrazolium ( MTT) assay, the apoptosis detected by Hoechst stain, the activity of superoxide dismutase( SOD) determined by nitrotetrazolium blue chloride ( NBT ) , and the level of malondialdehyde ( MDA ) detected by thiobarbituric acid ( TBA) . The expression of survivin gene in MCF-7 cells was test by RT-PCR. Results The proliferation of MCF-7 cells was inhibited by MSC, the cell viability reduced gradually with MSC concentration increased(P<0.01). In the MSC pretreatment group, it showed cell rounded, nuclear shrinkage, chromatin concentration by fluorescence staning. MSC could decrease the activity of SOD, and increase the level of MDA(P<0.01). The survivin gene ex-pression in MSC pretreatment group was lower than that of control group(P<0.01). Conclusion MSC can inhibit the proliferation of MCF-7 cells through regulating oxidative statues and the expression of survivin gene. MSC, a kind of new nutrition fortification, is being expected to be a new material to prevent and treat the breast cancer.关键词
硒-甲基硒代半胱氨酸/人乳腺癌MCF-7细胞/Sur-vivin基因/细胞凋亡Key words
Se-methylselenoeystein/human breast cancer MCF-7 cells/Survivin gene/cell apoptosis分类
医药卫生引用本文复制引用
罗雅婕,徐璐,邵继红,黄秋,谢蒙蒙..硒-甲基硒代半胱氨酸对MCF-7细胞抗氧化及Survivin基因表达的影响[J].安徽医科大学学报,2014,(2):194-197,4.基金项目
徐州市科技发展指导性计划(编号:XZZD1220) （编号:XZZD1220）
江苏省普通高校研究生科研创新计划项目(编号:CXZZ12_0998) （编号:CXZZ12_0998）
