安徽医科大学学报Issue(3)：291-294,295,5.
Aβ1-42寡聚体对AD大鼠大脑皮质Bcl-2、Caspase-3表达的影响
Effects of Aβ1-42 oligomer on cerebral cortical Bcl-2 and Caspase-3 expressions in AD rats
摘要
Abstract
Objective To investigate the changes in cortical Bcl-2 and Caspase-3 expression after microinfusion Aβ1-42 oligomers into right lateral cerebral ventricle. Methods After screening with Morris water maze, 60 rats with escape latency less than 60 s were randomly assigned equally into four groups: the naive group, PBS control group and Aβ1-42 fiber group and Aβ1-42 oligomers group. The rats in Aβoligomers and fiber group were infused Aβ1-42 oligomers or Aβ1-42 fiber into right cerebral ventricle to establish Alzheimer’s disease( AD) rat model;PBS control group received the same volume of PBS; naive group received no treatment. Four weeks after modeling, Morris water maze were performed to detect the changes in the ability of learning and memory in rats. RT-PCR as-say was used to detect the expression of cortical Bcl-2 mRNA and Caspase-3 mRNA in AD rats. Western blot assay was carried out to investigate cortical Bcl-2 protein expression and activity of Caspase-3 protein. Results Com-pared with naive and PBS control group, the escape latency in Aβ1-42 fiber group and Aβ1-42 oligomers group were prolonged than before modeling (P<0.05),the escape latency in Aβ1-42 oligomers group was longer than in Aβ1-42 fiber group;the cortical Bcl-2 mRNA expression in Aβ1-42 fiber and oligomers group were decreased( P<0.05 ) , and the Caspase-3 mRNA expression and activity were increased ( P<0.05 ) . The above-mentioned chan-ges of Bcl-2 and Caspase-3 mRNA expression in Aβ1-42 oligomers group were more prominent than that in Aβ1-42 fiber group. Conclusion Aβ1-42 fiber, Aβ1-42 oligomers can all lead to cognitive dysfunction, up-regulating Caspase-3 mRNA expression, increasing the activity of Caspase-3 protein and inhibiting Bcl-2 expression in AD rats, but the effects of Aβ1-42 oligomers on Caspase-3 and Bcl-2 mRNA expression are greater than in Aβ1-42 fi-ber.关键词
阿尔茨海默病/Aβ1-42 纤维体/Aβ1-42 寡聚体/Bcl-2/Caspase-3Key words
Alzheimer's disease/Aβ1-42 fiber/Aβ1-42 oligomers/Bcl-2/Caspase-3分类
医药卫生引用本文复制引用
陈雪,孙婧霞,曹翠丽,蒋常文..Aβ1-42寡聚体对AD大鼠大脑皮质Bcl-2、Caspase-3表达的影响[J].安徽医科大学学报,2014,(3):291-294,295,5.基金项目
国家自然科学基金(编号:81260174) （编号:81260174）
2012年广西研究生创新项目(编号:YCSW2012108) （编号:YCSW2012108）
