LY294002靶向抑制PI3K/Akt信号通路干预K562细胞增殖的研究OA北大核心CSTPCD
LY294002 targeting PI3 K/Akt signal pathway inhibit the proliferation of K562 cells
目的探讨PI3K/Akt抑制剂LY294002对慢性髓系白血病细胞株K562的增殖抑制作用及相关机制。方法MTT法检测LY294002对K562细胞增殖的抑制作用；10、20μmol/L LY294002作用K562细胞36 h,流式细胞术观察细胞周期的变化, RT-PCR 法测定 LY294002对 K562细胞Skp2基因表达的影响,Western blot法检测Skp2蛋白表达的变化。结果 LY294002能够抑制K562细胞的生长,该抑制…查看全部>>
Objective To explore the inhibition effects of LY294002(PI3K/Akt inhibition)on the proliferation and correlated regulatory factors of chronicmyeloid leukemia cell line K562 . Methods The effect of LY294002 on the proliferation of K562 cells were evaluated by MTT assays. K562 cells were cultured with 10,20 μmol/L LY294002 for 36 h. Flow cytometry analysis was used to determine the cell cycle. RT-PCR was applied to measure the expres-sion of S phase kinase ass…查看全部>>
耿英华;武文娟;于北凯;夏瑞祥
安徽医科大学第一附属医院血液内科，合肥 230022 蚌埠医学院第一附属医院血液内科，蚌埠 233004蚌埠医学院生物化学与分子生物学教研室，蚌埠 233030蚌埠医学院临床检验诊断学实验中心，蚌埠 233030
医药卫生
LY294002PI3K/AktSkp2K562
LY294002PI3 K/AktSkp2K562
《安徽医科大学学报》 2014 (3)
295-299,5
安徽省高等学校省级自然科学研究项目(编号:KJ2011B098)蚌埠医学院自然科学项目(编号:Byky1224)
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