按摩与康复医学Issue(4)：213-215,3.
杜仲苷对炎性环境下软骨细胞的增殖和II型胶原蛋白分泌的影响
The Influence of Aucubin on Proliferation of Cartilage Cells and Secretion of Collagen Type II under Inflammatory Environment
摘要
Abstract
Objective:To observe the influence of aucubin on the proliferation of vitro cartilage cells from IL-1βstimulated rats and their secretion of collagen type II. Methods:Using IL-1βstimulation to establish inflammatory model of vitro cartilage cells with rats, then divided into control group, IL-1β group, 10μMaucubin group, 100μMaucubin group, 500μMaucubin group and 1000μMaucubin group; 10% FBS medium was put in control group, while 10ng/mL IL-1β+10%FBS medium were in IL-1βgroup, and 10μM、100μM、500μM、1000μMaucubin+10ng/mL IL-1β+10%FBS medi-um were respectively in the four different concentration aucubin groups, all the groups were cultured for 48 hours, and used CCK8 to test prolifera-tion activity of cartilage cells and secretion of collagen type II. Results:The OD value of IL-1βgroup was lower than that of control group (P<0.05);the OD value of four different concetration aucubin groups were all higher than that of IL-1βgroup (P<0.05), and existed a dose-response relation-ship;the expression of collagen type II in the four different concentration aucubin groups were all higher than that of IL-1βgroup (P<0.05), and exist-ed a dose-response relationship. Conclusion:Aucubin can improve proliferation activity of rats' vitro cartilage cells under inflammatory environment, promote the secretion of collagen type II, which indicates that aucubin has certain anti-inflammatory effect on rats' vitro cartilage cells.关键词
杜仲苷/软骨细胞/IL-1β/炎性环境/增殖/II型胶原蛋白Key words
aucubin/cartilage cells/IL-1β/inflammatory environment/proliferation/collagen type II分类
医药卫生引用本文复制引用
谢国平,王胜楠,姜楠..杜仲苷对炎性环境下软骨细胞的增殖和II型胶原蛋白分泌的影响[J].按摩与康复医学,2014,(4):213-215,3.基金项目
广东省自然科学基金面上项目，编号S2011010004415 （）
