北京师范大学学报（自然科学版）Issue(1)：52-57,6.
电子束辐照金花葵幼苗的生物学效应及RAPD分析
BIOLOGICAL EFFECT AND RAPD ANALYSIS OF ELECTRON IRRADIATION ON ABELMOSCHUS MANIHOT
摘要
Abstract
Abelmoschusmanihot seedlings were irradiated by electron beam at doses of 0,30 and 50 Gy. Survival rate of Abelmoschus manihot was influenced by electron beam irradiation.Growth was examined and genetic variation was studied by RAPD analysis.Plants irradiated by election beam showed a dwarf phenotype, plants irradiated at 50 Gy were lower than at 30 Gy.The reproductive growth of plants such as blossom, fructification and seed maturation were influenced,and such effect increased with doses;with irradiation at 50 Gy,plants stopped seeding all together.Eight appropriate random primers were screened:S10,S101,S168, S198,S217,S223,S248 and S249.RAPD analysis showed that genetic distance between control group and 30 Gy group,50 Gy group was 0.284 0 and 0.376 6 used leaves as experimental material.Genetic distance between controls seed and 30 Gy irradiated seed was 0.103 4.关键词
电子束辐照/生物学效应/RAPD/金花葵Key words
electron beam irradiation/biological effect/RAPD/Abelmoschus manihot引用本文复制引用
何丽娴,王阳,杨欢,张丰收,张涛,苏颖,连慕兰,周云龙..电子束辐照金花葵幼苗的生物学效应及RAPD分析[J].北京师范大学学报（自然科学版）,2014,(1):52-57,6.基金项目
射线束技术与材料改性教育部重点实验室基金资助项目(201123) （201123）
