滨州医学院学报Issue(6)：401-404,4.
褐藻多糖硫酸酯对 TGF-β1体外诱导的肾小管上皮细胞间质转分化过程中细胞骨架的影响
Effects of Fucoidan on the cytoskeleton during the course of epithelial mesenchymal transition induced by TGF-β1 of human renal tubular epithelial cell in vitro
摘要
Abstract
Objective To observe the Fucoidan in vitro against TGF-β1-induced renal tubular epithelial cells ( HK-2 ) mesenchy-mal transition process cytokeratin (cytokeratin,CK) and α-smooth muscle actin (α-smooth muscle action,α-SMA) expression,and to explore the Fucoidan on HK-2 epithelial-mesenchymal transition and its mechanism .Methods We cultured cell lines of normal human renal tubular epithelial cells (HK-2).Using an inverted phase contrast microscope ,we observed morphological changes of cells in con-trol group,induction group and drug group .With the use of immune cells SABC method we cultured HK-2 cells CK andα-SMA-positive cells of the control group ,the induction group and the drug group for 3 days and detected their average optical density values with image analysis .Results After three days of culture ,HK-2 cells showed cobblestone morphology characteristic of epithelial cells in the control group,long fusiform in the induction group ,which was similar to fibroblasts,and mainly epithelial cells over cells in the drug group ,with a few cells fusiform.Compared with the control group ,the average optical density values of CK-positive cells in the induced group were significantly lower (P<0.05),which those of α-SMA-positive cells increased (P<0.05).Compared with the induction group the av-erage optical density values of CK-positive cells in the drug group were significantly incerased ( P<0.05 ) , Wile those of α-SMA-posi-tive cells were significantly lower (P<0.05).Conclusion ①Fucoidan inhibits vitro mesenchymal transdifferentiation of TGF-β1-in-duced renal tubular epithelial cells;②Fucoidan may play a part inhibiting mesenchymal transdifferentiation of renal tubular epithelial cells by altering the cytokeleton .关键词
褐藻多糖硫酸酯/TGF-β1/肾小管上皮细胞/转分化/细胞角蛋白/α-平滑肌肌动蛋白Key words
Fucoidan/TGF-β1/Human renal tubular epithelial cell/Epithelial mesenchymal transition/Cytokeratin/α-smooth mus-cle action分类
医药卫生引用本文复制引用
于国华,王威,冯国营,曲桂梅,姚卫东,郎志强,孔令玲..褐藻多糖硫酸酯对 TGF-β1体外诱导的肾小管上皮细胞间质转分化过程中细胞骨架的影响[J].滨州医学院学报,2013,(6):401-404,4.基金项目
山东省中医药管理局科技发展计划资助课题 （）
