磁共振成像Issue(6)：431-436,6.DOI:10.3969/j.issn.1674-8034.2013.06.007
7.0TMRI体外标记Gd-DTPA对骨髓间充质干细胞的实验研究
Study of Gd-DTPA labeled bone marrow mesenchymal stem cells in vitro with 7.0 T MRI
摘要
Abstract
Objective:The aim of this study was to explore the possibility of the rat bone mesenchymal stem cells (BMSCs) labeled with standard contrast agents (Gd-DTPA) for stem cell tracking. Materials and Methods:BMSCs from bilateral femur of rats were cultured and propagated. The BMSCs uptaked Gd-DTPA. Electron microscopy was performed to detect the distribution of Gd-DTPA particles in BMSCs. Viability and proliferation of labeled BMSCs were evaluated using trypan blue exclusion assays and MTT assay. Labeled BMSCs were detected with T1-weighed MR imaging in vitro. Results:The presence of Gd-DTPA particles inside the BMSCs was deifnitely detected by transmission electron microscopy. There was no difference in viability and proliferation between the labeled and unlabeled cells. The labeled BMSCs demonstrated the high signal intensity on T1-weighted MRI in vitro. Conclusion:Gd-DTPA shows no evident adverse effect on the function of labeled BMSCs. Gd-DTPA can be used for the MR imaging tracking of labeled BMSCs.关键词
间质干细胞/磁共振成像/染色与标记/钆DTPA/动物,实验Key words
Mesenchymal stem cells/Magnetic resonance imaging/Staining and labeling/Gadolinium DTPA/Animals, laboratory分类
医药卫生引用本文复制引用
耿宽,贾岩龙,黄得校,易美芝,杨忠现,林艳,吴仁华..7.0TMRI体外标记Gd-DTPA对骨髓间充质干细胞的实验研究[J].磁共振成像,2013,(6):431-436,6.基金项目
国家自然科学基金重点项目(编号30930027)；国家自然科学基金青年基金（编号81101102）；广东省自然科学基金 （编号30930027）
