磁共振成像Issue(6)：437-440,4.DOI:10.3969/j.issn.1674-8034.2013.06.008
9.4TMR波谱检测间充质干细胞成骨分化代谢特征的研究
9.4 T MR spectroscopy detection of mesenchymal stem cells into osteoblasts metabolism characteristics
摘要
Abstract
Objective: To explore the metabolite profile of mesenchymal stem cells (MSCs) underwent osteogenic differentiation using 9.4 T high resolution MR spectroscopy. Materials and Methods: Human umbilical cord mesenchymal stem cells were cultured and collected. MSCs were induced osteogenic differentiation for 2 weeks and cell metabolite extractions were prepared by methanol-chloroform (M/C) method. Cell extractions were then analyzed on a 9.4 T MRS device. Results:After osteogenic inducement, the fatty acid metabolite peak at 2.02 ppm and 2.25 ppm obviously decreased, and there appeared a new metabolite peak at about 1.77 ppm, which is most probably representative of leucine. Conclusions:There are some speciifc characteristics on MRS of MSCs underwent osteogenic differentiation. The new arised peak at 1.77 ppm may serve as the biomarker for mesenchymal stem cells osteogenic differentiation.关键词
间质干细胞/磁共振波谱学/成骨细胞/代谢Key words
Mesenchymal stem cells/Magnetic resonance imaging/Staining and labeling/Gadolinium DTPA/Animals, laboratory分类
医药卫生引用本文复制引用
洪璧楷,代海洋,肖叶玉,吴仁华..9.4TMR波谱检测间充质干细胞成骨分化代谢特征的研究[J].磁共振成像,2013,(6):437-440,4.基金项目
国家自然科学基金重点项目(30930027)；广东省医学科研项目(A2011405) （30930027）
