重庆医学Issue(26)：3084-3086,3.DOI:10.3969/j.issn.1671-8348.2013.26.002
氟尿嘧啶缓释剂植入对人结直肠癌裸鼠移植瘤生长抑制的观察
Observation on growth inhibition of sustained-release fluorouracil implantation in nude mice with human colorectal carcinoma
摘要
Abstract
Objective To observe the growth inhibition effect of sustained-release fluorouracil implantation in nude mice with human colorectal carcinoma and to discuss its influence on caspase-3 expression .Methods The colorectal carcinoma subcutaneous transplanted model with LoVo cell in BALB/c nude mice was established and divided into 4 groups randomly :the tail intravenous injection of PBS (A group) ,the tail intravenous injection of 5-FU group (B group) ,the intratumor injection of 5-FU group (C group);and the implant of sustained-release fluorouracil group(D group) .After 15 d of medication ,the tumor weight and tumor volume were recorded ,the tumor inhibiting rates were calculated .The tumor tissues were observed by HE staining and detected the expression of caspase-3 by immunohistochemistry (IHC) .Results Sustained-release fluorouracil could suppress the transplantation tumor growth significantly ,the tumor weight and tumor volume in the D group had statistical differences compared with the A ,B and C groups(P<0 .05) ,which could up-regulated the expression of caspase-3 ,the differences between the D group with the A ,B and C groups (P<0 .05) .Conclusion Sustained-release fluorouracil can inhibit the grow th of colorectal carcinoma transplantation tumor more effectively than conventional fluorouracil and up-regulate the expression of caspase-3 ,which prompts that sustained-re-lease fluorouracil may play the anti-tumor role by inducing the tumor cell apoptosis via apoptosis pathway .Its definite tumor-inhibi-ting effect deserves to be promoted in clinical chemotherapy .关键词
结直肠肿瘤/氟尿嘧啶缓释剂/半胱氨酸天冬氨酸特异性蛋白酶-3Key words
colorectal neoplasms/sustained-release fluorouracil/caspase-3引用本文复制引用
李弘,颜丽萍,石晓萍..氟尿嘧啶缓释剂植入对人结直肠癌裸鼠移植瘤生长抑制的观察[J].重庆医学,2013,(26):3084-3086,3.基金项目
广西壮族自治区桂林市科技攻关与新产品试制科研基金（20110119-5）。 （）
