重庆医学Issue(26)：3125-3126,3128,3.DOI:10.3969/j.issn.1671-8348.2013.26.018
多发性骨髓瘤染色体异常的荧光原位杂交技术检测
Detection of chromsomal abnormalities in patients with multiple myeloma by fluorescence in situ hybridization
摘要
Abstract
Objective To investigate the common chromosomal abnormalities and clinical significance of the patients with multi-ple myeloma(MM) by fluorescence in situ hybridization FISH ) .Methods By using FISH ,five specific probes were used to detect the chromosomal abnormalities of 18 patients with MM .Results Among 18 cases of MM ,there were 13 cases of chromosome ab-normalities ,the overall detection rate of chromosomal abnormalities was 72% (13/18) .And the frequencies of abnormalities in lq21 , the deletion of chromosome 13(including D13S319 and RB-1 two probes) ,translocation involving the 14q32 region ,and deletion of chromosome 17 were 6% (1/18) ,17% (3/18) ,17% (3/18) ,33% (6/18) ,6% (1/18) .Analysis showed that ,there was no correlation between the incidence of chromosomal abnormalities and that the sex ,age ,D-S stage ,ISS stage ,hemoglobin ,platelets ,plasma cells ,β2-MG ,albumin ,creatinine ,lactate dehydrogenase ,C-reactive protein in the patients with MM .MM patients with chromosome 13 deletion ,the treatment effect was poor .Conclusion The FISH technology for detecting genetic abnormalities is more sensitive and reliable .The deletion of chromosome 13 and the translocation involving the chromosome 14 is more common .There is no correlation between the clinical features of MM patients and their chromosomal abnormalities .关键词
多发性骨髓瘤/染色体/原位杂交 ,荧光Key words
multiple myeloma/chromosomes/in situ hybridization ,fluoresence引用本文复制引用
田祖国,袁钟,翁春岚..多发性骨髓瘤染色体异常的荧光原位杂交技术检测[J].重庆医学,2013,(26):3125-3126,3128,3.基金项目
卫生部科研基金资助课题（W K J2007-3-001）。 （）
