重庆医学Issue(26)：3139-3141,3.DOI:10.3969/j.issn.1671-8348.2013.26.024
AcSDKP对硅肺纤维化大鼠胶原含量及NF-κBp65表达的影响
Efect of AcSDKP on collagen content and expression of NF-κB p65 in lung of rats with silicosis fibrosis
摘要
Abstract
Objective To investigate the effect of N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP) on the synthesis of collagen and the expression of NF-κB p65 in lung of rats with silicosis fibrosis .Methods Rats were divided into 3 groups randomly :the control group(each rat was intratracheally instilled with 1 mL normal saline and killed after 8 weeks ,the silicotic model group(each rat was intratracheally instilled with 1 mL silica suspension and killed after 8 weeks ,the AcSDKP treated group(each rat was intra-tracheally instilled with 1 mL silica suspension and AcSDKP (800 μg · kg -1 · d-1 ) was administered into every rat ,then rats were killed after 8 weeks .Lung fibrosis in morphology and collagen content was observed by HE and vg staining and hydroxyproline as-say .The expression of NF-κB p65 was detected with the immunohistochemistry and Western blot .Results Compared with the con-trol group ,the quantity of silicon nodules ,collagen content and the expression of NF-κB p65were increased in the silicotic model group .Compared with the silicotic model group ,the quantity of silicon nodules ,collagen content and the expression of NF-κB p65 were decreased in the AcSDKP-treated group .Conclusion AcSDKP could inhibit the activity and expression of NF-κB p65 by strengthening the stability of I-κB ,which may influence the progress of alveolar inflammation and silicosis fibrosis .关键词
矽肺/N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸 ,核转录因子κB p65Key words
silicosis/N-acetyl-seryl-aspartyl-lysyl-proline/NF-κB p65引用本文复制引用
闫静波..AcSDKP对硅肺纤维化大鼠胶原含量及NF-κBp65表达的影响[J].重庆医学,2013,(26):3139-3141,3.基金项目
邢台市科学技术研究与发展计划项目（2012ZC181）。 （）
