重庆医学Issue(28)：3347-3349,3.DOI:10.3969/j.issn.1671-8348.2013.28.005
GSK-3β、PTEN 、PLK1在儿童急性髓系白血病中的表达及意义
Expression and clinical significance of GSK-3β,PTEN,PLK1 in pediatric acute myeloid leukemia
王妍 1徐酉华 1胡艳妮 1孙艳辉 1金鑫 1杨桂存1
作者信息
- 1. 重庆医科大学附属儿童医院/儿童发育疾病研究省部共建教育部重点实验室/儿科学重庆市重点实验室/重庆市儿童发育重大疾病诊治与预防国际科技合作基地 400014
- 折叠
摘要
Abstract
Objective To explore the expression and clinical significance of GSK-3β,PTEN and PLK1 in pediatric AML . Methods Experiment group was bone marrows of 33 cases incipient children with AML .Control group was 10 cases normal bone marrows .GSK-3β,PTEN and PLK1 gene expressions in BMMNC of the two groups were tested using semi quantitative reverse transcriptase polymerase chain reaction(RT-PCR)technique .GSK-3βprotein and P-GSK-3βexpressions were tested by ELISA .Re-sults The expression of GSK-3βmRNA ,GSK-3βprotein ,PLK1 mRNA in BMMNC of children with AML was higher than that of control group(P=0 .012 ;P= 0 .014 ;P= 0 .040);The expression of PTEN mRNA ,P-GSK-3β was lower than the control group (P=0 .012 ;P=0 .002);GSK-3βprotein had a negative correlation with PTEN mRNA (r= -0 .415 ,P=0 .016);GSK-3βmRNA , GSK-3βprotein had a positive correlation with PLK 1 mRNA(r=0 .388 ,P=0 .026;r=0 .427 ,P=0 .013) .The expression of GSK-3βprotein was high in which had high peripheral white blood cell counts ;both the expressions of GSK-3βmRNA and GSK-3βpro-tein were high in which had high risk ;but the expression of P-GSK-3βwere low .Conclusion In pediatric AML ,GSK-3βand PLK1 may play a role of oncogene and PTEN may play a role of tumor suppressor gene .关键词
GSK-3β/PTEN/PLK1/前体细胞淋巴母细胞白血病淋巴瘤Key words
GSK-3β/PTEN/PLK1/precursor cell lymphoblastic leukemia-lymphoma引用本文复制引用
王妍,徐酉华,胡艳妮,孙艳辉,金鑫,杨桂存..GSK-3β、PTEN 、PLK1在儿童急性髓系白血病中的表达及意义[J].重庆医学,2013,(28):3347-3349,3.
