重庆医学Issue(28)：3405-3407,3.DOI:10.3969/j.issn.1671-8348.2013.28.028
右美托咪定预处理对急性肺损伤大鼠的保护作用
The protective effect of dexmedetomidine preconditioning on acute lung injury in rats
葛东建 1祁宾 1李金玉1
作者信息
- 1. 南京医科大学附属淮安第一医院麻醉科，江苏淮安223300
- 折叠
摘要
Abstract
Objective To investigate the role of Akt signaling in the protective effect of dexmedetomidine preconditioning on the lipopolysaccharide(LPS)-induced acute lung injury (ALI) in rat .Methods Twenty-two SD rats were randomly divided into 4 groups:before LPS injection group(T0 group) ,1 h after LPS injection group(T1 group) ,3 h after LPS injection group(T2 group) and 6 h after LPS injection group(T3 group) ,the expression of Akt and p-Akt in the process of LPS-induced ALI was investigated . Another 54 rats were divided into 3 groups :control group(C group) ,6 hours of ALI group(ALI group) and Dexmedetomidine+ALI group(D+ ALI group) ,the expression of p-Akt in lung tissues ,the protein concentration in bronchoalveolar lavage fluid (BALF) ,apoptotic index and the pathological changes in the lung tissues were observed .Forty rats were divided into ALI group and ALI + D group to investigate the 48 h-survival ratio .Results Compared with T0 group ,the level of p-Akt expression in T1 ,T2 , T3 groups were deceased in a time-dependent manner(P<0 .05) .Compared with ALI group ,p-Akt in D + ALI group increased significantly(P<0 .05) but was still lower than that in C group(P<0 .05);the protein concentration in BALF and the apoptotic in-dex in D + ALI groups were significantly lower than those in ALI group(P<0 .05) ,but still higher than those in C group(P<0 .05) .The 48 h survival ratio was significantly decreased in D + ALI group comparing with ALI group(P<0 .05) .Conclusion Dexmedetomidine preconditioning might display protective effect via activating p-Akt signaling pathway in LPS-induced ALI .关键词
右美托咪定/急性肺损伤/脂多糖类/p-AktKey words
dexmedetomidine/acute lung injury/lipopolysaccharides/p-Akt引用本文复制引用
葛东建,祁宾,李金玉..右美托咪定预处理对急性肺损伤大鼠的保护作用[J].重庆医学,2013,(28):3405-3407,3.
