重庆医学Issue(29)：3468-3471,4.DOI:10.3969/j.issn.1671-8348.2013.29.002
印迹基因 H19对人绒毛膜上皮癌细胞JEG-3基因表达谱的影响
The effect of imprinting gene H19 on the gene expression profile of human choriocarcinoma cell line JEG-3
摘要
Abstract
Objective To obtain the expression pattern of imprint gene H19 in JEG-3 cell in order to explore the regulation mechanism of H19 on trophoblast cellular biological behavior .Methods After correct identification with sequencing for the recom-binant eukaryotic expression plasmid pRc/CMV which including the whole length of H19 cDNA ,the plasmid was transfected to the cell line JEG-3 .The expression of H19 mRNA was observed and the gene expression profile of three groups of JEG-3 cell were de-tected with Affymetri :U133 plus 2 .0 Array .Results After being transfected with target H 19 gene ,the expression of the mRNA level was significantly increased compared with control group .And the gene expression profile was changed significantly .19 genes were up-regulated ,77 genes were down-regulated .Expression levels of HES1 gene which being choosed as a different expression gene were detected by fluorescence quantitative PCR in severe preeclampsia placenta tissue and normal late pregnant placenta .The expression level of HES1 mRNA in severe preeclampsia placenta decreased significantly than normal late pregnant placenta tissues . Conclusion Many genes induced by H19 have been screened by high-throughput gene chip method .It provides the experimental ba-sis for advanced studying the regulation the cellular biological behavior with H 19 gene .关键词
H19/滋养细胞/基因芯片/子痫前期/HES1Key words
H19 gene/trophoblast/gene chip/pre-eclampsia/HES1引用本文复制引用
俞丽丽,李力,赵丹,卢林杉,郑英如,陈星云,李平,周元国..印迹基因 H19对人绒毛膜上皮癌细胞JEG-3基因表达谱的影响[J].重庆医学,2013,(29):3468-3471,4.基金项目
国家自然科学基金资助项目（81070505）。 （）
