重庆医学Issue(32)：3929-3931,3934,4.DOI:10.3969/j.issn.1671-8348.2013.32.031
仙鹤草水提液对SMMC-7721肝癌细胞的抗癌作用及其机制
Anti-tumor effects of Agrimonia Pilosa Ledeb .on SMMC-7721 hepatocellular carcinoma cells and its mechanisms
邹夏慧 1张焜和 1陈江 1黄德强 1祝金泉 1朱萱 1吕农华1
作者信息
- 1. 南昌大学第一附属医院消化内科/江西省消化疾病研究所，南昌330006
- 折叠
摘要
Abstract
Objective To observe the anti-tumor effects of Agrimonia Pilosa Ledeb(APL) ,a Chinese herbal medicine ,on hepato-cellular carcinoma cells in vitro and investigate the underlined mechanisms preliminarily .Methods APL water extracts were pre-pared .SMMC-7721 cells were cultured with the medium containing different concentrations of APL water extracts ,and at different time points ,cell viabilities were measured by the MTT assay and inhibitory rates (IR) were calculated ;cell morphologic changes were observed under a light microscope ;apoptotic ratios were measured by flow cytometry ;and the expressions of Bcl-2 and P53 proteins were examined by immunocytochemistry .Results After the cells were cultured with the medium containing APL water ex-tracts for 24 h ,48 h and 72 h ,no obvious effects were found on the cell proliferation in 5 mg/mL group and 10 mg/mL group ,but IR were 0 .5% ,23 .9% and 27 .5% in 20 mg/mL group and 23 .3% ,51 .7% and 71 .6% in the 40 mg/mL group ,respectively .In the groups with effects on the cells proliferation ,morphological characteristics of apoptosis were obvious ,and the cell apoptotic ratios were 19 .5% and 23 .0% in 20 mg/mL group and 33 .4% and 42 .7% in 40 mg/mL group at 48 h and 72 h .The expressions of Bcl-2 protein were 71 .9% and 58 .5% in 20 mg/mL group and 47 .9% and 26 .5% in 40 mg/mL group at 48 h and 72 h ,and the ex-pressions of P53 protein were 22 .9% and 50 .6% in 20 mg/mL group and 48 .7% and 83 .7% in 40 mg/mL group at 48 h and 72 h . Conclusion The water extracts of APL are able to inhibit proliferation and induce apoptosis of SMMC-7721 cells dose-time depend-ently in vitro ,which might be associated with the expression changes of Bcl-2 and P53 protein .关键词
肝肿瘤/细胞凋亡/仙鹤草Key words
liver neoplasms/apoptosis/Agrimonia Pilosa Ledeb引用本文复制引用
邹夏慧,张焜和,陈江,黄德强,祝金泉,朱萱,吕农华..仙鹤草水提液对SMMC-7721肝癌细胞的抗癌作用及其机制[J].重庆医学,2013,(32):3929-3931,3934,4.
