重庆医学Issue(33)：3977-3979,3.DOI:10.3969/j.issn.1671-8348.2013.33.001
G蛋白偶联受体激酶4突变A142V基因对大鼠血管平滑肌细胞AT1受体的影响
Regulation of human G-coupled protein kinase 4 A142V on AT1 receptor in rat vascular smooth muscle cells
摘要
Abstract
Objective To study the effect of human G-coupled protein kinase 4(GRK4) A142V overexpression on angiotensin Ⅱ1 type(AT1 ) receptor and its-mediated proliferation of rat vascular smooth muscle cells .Methods We constructed a lentiviral vec-tor carrying human GRK4-EGFP gene and observed its expression in A10 cells .Expression of AT1 receptor were determined by im-munoblotting ,GRK4 activity were checked by spectrophotometry ;the linkage between GRK4 and AT1 receptor were determined by co-immunoprecipitation .[3 H] thymidine incorporation was used to detect changes of cell proliferation .Results As compared with the control cells ,A142V-transfected cells had higher GRK4 activity and higher AT1 receptor expression ;there was linkage between GRK4 and AT1 receptor ,the co-immunoprecipitation levels were lower in A142V cells .The basal levels of VSMC proliferation was higher in A142V cells ,Ang Ⅱ increased VSMC proliferation to a greater extent in A 142V cells .Conclusion GRK4 A142V ,via in-creasing GRK4 activity ,increases AT1 receptor expression and function in vascular smooth muscle cell proliferation .关键词
高血压/G蛋白偶联受体激酶4/受体 ,血管紧张素 ,1型/血管平滑肌细胞Key words
hypertension/G-protein-coupled receptor kinase 4/receptor ,angiotensin ,typel/vascular smooth muscle cells引用本文复制引用
邓昆,刘莉,陈彩宇,陈垦,王微,周永巧,何多芬,曾春雨..G蛋白偶联受体激酶4突变A142V基因对大鼠血管平滑肌细胞AT1受体的影响[J].重庆医学,2013,(33):3977-3979,3.基金项目
国家杰出青年科学基金资助项目（30925018）；国家自然科学基金资助项目（31130029，81070559，81100500）。 （）
