重庆医学Issue(33)：4056-4058,4061,4.DOI:10.3969/j.issn.1671-8348.2013.33.032
PAR-1激活对小鼠内皮祖细胞CXCR4 mRNA 表达及细胞增殖与迁移的影响
Effect of activated PAR-1 on the expression of CXCR4 mRNA,proliferation and migration of mouse endothelial progenitor cells
摘要
Abstract
Objective To explore the effect of protease-activated receptor 1(PAR-1) activation on the expression of CXCR4 mR-NA of mouse endothelial progenitor cells (EPCs) and proliferation ,migration of EPCs .Methods Mouse EPCs were activated by different concentration of SFLLRN (one agonist of PAR-1) ,or transfected by small interfering RNA of PAR-1 .The expressions of PAR-1 and CXCR4 mRNA of EPCs were detected by fluorescent quantitative real-time PCR .Proliferation ,migration of EPCs were detected by MTT ,Transwell chambers respectively .Results The expressions of PAR-1 and CXCR4 mRNA in SFLLRN group were higher than that in other groups(P< 0 .05) .The expression of CXCR4 mRNA was highly positive correlation with PAR-1 mRNA(r=0 .991) .The proliferation ,migration of mouse EPCs were induced by activation of PAR-1 .Conclusion Activation of PAR-1 promotes cell proliferation and migration of mouse EPCs ,this effect may be depended on CXCR4 .关键词
内皮祖细胞/蛋白酶激活受体-1/CXC型趋化因子受体4/细胞增殖/迁移Key words
endothelial progenitor cells/protease-activated receptor 1/CXC receptor 4/cell proliferation/migration引用本文复制引用
代静澜,潘波,古平,牟海刚,白家驷..PAR-1激活对小鼠内皮祖细胞CXCR4 mRNA 表达及细胞增殖与迁移的影响[J].重庆医学,2013,(33):4056-4058,4061,4.基金项目
解放军第三二四医院院管课题资助项目（2010008）。 （）
