重庆医学Issue(34)：4112-4114,3.DOI:10.3969/j.issn.1671-8348.2013.34.003
APE1／Ref-1在外周血单核细胞破骨样分化中的作用研究
Effect of APE1/Ref-1 on differentiation of peripheral blood mononuclear cells into osteoclast-like cells
摘要
Abstract
Objective To investigate the effect of APE1 on differentiation of peripheral blood mononuclear cells into osteoclast-like cells(OCL) which induced by macrophage colony stimulating factor (RANKL) and macrophage colony stimulating factor (M-CSF) .Methods Human peripheral blood mononuclear cells (PBMCs) were collected by density gradient separation ;Constructed APE1 siRNA expression vector Ad5v-APE1 siRNA was used to transfect PBMCs .Tartrate-resistant acid phosphatase (TRAP) method was conducted to identify the cells ,the expression level of APE1 was detected by Western blot ,the mRNA expression levels of Cathepsin K(CK) and V-ATPase were detected by RT-PCR .Results PBMCs transfected with APE1 siRNA had significantly lower protein expression of APE1 than untransfected cells (P< 0 .05) ;PBMCs could differentiate into OCL under the stimulation of RANKL and M-CSF ,the mRNA expression levels of CK and V-ATPase increased ;After APE1 siRNA treatment ,the number of OCL was reduced and the levels of CK and V-ATPase mRNA decreased .Conclusion PBMCs can differentiate into a large number of OCL induced by RANKL and M-CSF ,APE1 siRNA significantly inhibited differentiation of PBMCs into osteoclast-like cells , APE1 may be involved in the regulation of osteoclast-like differentiation process .关键词
单核细胞/细胞分化/破骨细胞/脱嘌呤/脱嘧啶核酸内切酶Key words
mononuclear cells/osteoclast cells/cell differentiation/apurinic/apyrimidinic endonuclease/redox-factor1引用本文复制引用
杜佳,谢家印,张亮,周立为,杨宇馨,王东..APE1／Ref-1在外周血单核细胞破骨样分化中的作用研究[J].重庆医学,2013,(34):4112-4114,3.基金项目
国家自然科学基金资助项目（81172258）。 （）
