重庆医学Issue(1)：12-15,4.DOI:10.3969/j.issn.1671-8348.2014.01.004
HPV L1 C-末端保守序列多肽免疫学特性的探讨
To investigate the immunity characteristics of a conserved sequence on the C terminal region of the human papillomavirus major protein L 1
摘要
Abstract
Objective To investigate and detect the immunity characteristics of a conserved sequence including 30 amino residues which is located in the C terminal of HPVL1 .Methods The immune model of mouse was establish with a polypeptide synthetized based on this sequence .The amount of CD3+ ,CD3+CD4+ or CD3+CD8+ lymphocyte of the mouse spleen was detected by Flow cy-tometry ,then the value of CD4+ /CD8+ were calculated .The cell proliferation was detected by MTT assay .Sample was took from the cell supernatant to detect the content of IL-4 and IFN-γby double antibody sandwich ELISA .Results (1)The amount of CD3+CD8+ lymphocytes and the value of CD4+ /CD8+ were significantly increased than control group(P<0 .05) .(2)The result of T cell proliferation showed no significant difference (P>0 .05) .(3)The level of IL 4 were significantly higher than that of control group (P<0 .01) .The content of IFN-γlevel showed no significant difference compared with control group (P>0 .05) .Conclusion The results showed that it was affirmative that the polypeptides induced humoral immunity ,which was worth to be furtherly studied as a preventive vaccine .But its cell immunogenicity is weak ,and the attenmpt to induce the response related to the cell immunogenicity was failed ,and the immunogenicity of the peptide remains to be improved .关键词
HPV/晚期蛋白/保守序列/免疫原性Key words
HPV/late protein/conserved sequence/immunogenicity引用本文复制引用
蒋雪梅,肖长义,吴江峰,李红军,袁太宁,汤桂成..HPV L1 C-末端保守序列多肽免疫学特性的探讨[J].重庆医学,2014,(1):12-15,4.基金项目
国家自然科学基金资助项目（30770104）；湖北省教育厅项目（B20121306）；三峡大学人才科研启动基金项目（K J2010B045）。 （）
