重庆医学Issue(3)：270-272,3.DOI:10.3969/j.issn.1671-8348.2014.03.005
白念珠菌耐药基因CDR1、CDR2、M DR1表达与氟康唑耐药的相关性分析
Analysis on correlation between Candida albicans clinical isolates resistance genes CDR1,CDR2,MDR1 expression with fluconazole resistance
摘要
Abstract
Objective To explore the expression level of Candida albicans fluconazole resistance genes CDR1 ,CDR2 and MDR1 in susceptible and resistant strains .Methods Candida albicans was isolated from the detection samples and identified ,115 strains were selected and determined MIC of Candida albicans by the microdilution method .Then PCR was adopted to detect the expression level of Candida albicans resistance genes in susceptible and resistant strains .Results The half inhibitory range in 115 strains of Candida albicans to fluconazole was 5 μg/mL ,90% of antibacterial range was 126μg/mL ;among them ,76 strains were sensitive ,39 strains were resistant ,the sensitivity rate was 66 .1% ,the resistant rate was 36 .8% .The CDR2 gene △ CT was 9 .33 ± 3 .21 for the sensitive strains and 7 .49 ± 2 .54 for the resistant strains ,two groups had statistically significant difference (t=2 .659 ,P<0 .05);the expression levels of CDR1 gene and MDR1 gene had no significant difference between the susceptible strains and the resistant strains(P>0 .05) .Conclusion Drug resistance of Candida albicans is more complicated process ,in which ,its resistance to flucon-azole is related with the high expression of CDR2 and has no relation with the expression of CDR1 and MDR1 .关键词
念珠菌 ,白色/耐药基因/氟康唑/相关性Key words
Candida albicans/drug resistance gene/fluconazole/relevance引用本文复制引用
邵俊国,魏媛媛,张金艳,冯鑫,张洪涛,刘志广,李筱芳..白念珠菌耐药基因CDR1、CDR2、M DR1表达与氟康唑耐药的相关性分析[J].重庆医学,2014,(3):270-272,3.基金项目
国家自然科学基金资助项目（81101203）。 （）
