重庆医学Issue(5)：569-571,3.DOI:10.3969/j.issn.1671-8348.2014.05.022
缺氧诱导滋养细胞自噬参与子痫前期发病的实验研究
Experimental study of hypoxia-inducible trophoblastic autophagy involved in the pathogenesis of preeclampsia
摘要
Abstract
Objective To study hypoxia environment trophoblast cell autophagy and autophagy of the preeclampsia placental tis-sue .Methods Trophoblastic cell line HTR-8/SVneo were divided into three groups :low oxygen concentration group (group Ⅰand group Ⅱ) and normal oxygen concentration group ;48 h after the application the confocal laser scanning microscopy was used for detection of cytoplasmic autophagosome ,PCR technology analysis of autophagy-related gene expression change of LC3-Ⅱ .LC3-Ⅱ expression levels of 30 cases of preeclampsia placenta were detected by Immunohistochemistry .Results In low oxygen concen-tration group ,there were visible red autophagosome chromatin structure in cytoplasm ,which was extremely rare in the normal oxy-gen concentration group ,in low oxygen concentration group cell LC3-Ⅱ mRNA expression was significantly higher than that of normal concentration group .The preeclampsia placenta of patients with positive immunostaining of LC 3-Ⅱ was 67 .12% ,compared with 9 .14% in the normal control ,there was a significant difference between two groups .Conclusion Preeclampsia placenta auto-phagy activity increased ,hypoxia can induce autophagy trophoblast cell line phenomenon .关键词
自噬/缺氧/子痫前期Key words
autophagy/anoxia/preeclampsia引用本文复制引用
秦兴发,张华,漆洪波,陈国庆,马海霞..缺氧诱导滋养细胞自噬参与子痫前期发病的实验研究[J].重庆医学,2014,(5):569-571,3.基金项目
国家自然科学基金资助项目（81070502）；国家临床重点专科建设项目经费资助。 （）
