重庆医学Issue(5)：589-591,3.DOI:10.3969/j.issn.1671-8348.2014.05.029
FimH1-156融合基因的构建及其原核蛋白表达与纯化
Construction,prokaryotic expression and purification of FimH1-156 fusion protein
摘要
Abstract
Objective To construct and express a prokaryotic expression vector carrying the gene of FimH 1-156 that comprises human lysosome membrane protein 2 P41-49 gene ,and to express and purify the fusion protein .Methods FimH1-156 gene was cloned from plasmid pPKL241 by PCR ,and inserted into vector pET-28a(+ ) to obtain prokaryotic expression plasmid pET-28a-FimH . After transforming Escherichia coli BL21(DE3) with pET-28a-FimH ,fusion protein FimH1-156 was expressed under induction .The target fusion protein was purified ,and its antigenicity was detected through Western blot .Results The expressed recombinant pro-tein was purified ,the expression of protein was the highest when IPTG was 1 mmol/L and 4h after induction ,it was expressed as include body form ,and the expressed protein was identified to react with monoclonal antibodies 6 × His by Western blotting .Conclu-sion We cloned FimH1-156 fusion protein expressed genes successfully ,constructed prokaryotic expression vector ,and won the in-clusion body purification of FimH1-156 fusion protein .关键词
Fim H1 -156/融合蛋白/表达/纯化Key words
FimH1 -156/fusion protein/expression/purification引用本文复制引用
尹仕伟,邹丽云,张莹,张晋宇,唐莎,施维维,吴玉章,袁发焕,张静波..FimH1-156融合基因的构建及其原核蛋白表达与纯化[J].重庆医学,2014,(5):589-591,3.基金项目
国家自然科学基金资助项目（30971366）；重庆市国际合作项目（CSTC201110004）；第三军医大学科研课题（2011XLC37）。 （）
