重庆医学Issue(7)：769-771,778,4.DOI:10.3969/j.issn.1671-8348.2014.07.001
铜绿假单胞菌对致病真菌的抑制作用
The inhibiting effect of pseudomonas aeruginosa to the growth of pathogenic fungi
摘要
Abstract
Objective To explore the inhibitory effect of 24 pseudomonas aeruginosa(PA) on pathogenic fungi ,such as candida albicans ,candida tropicalis ,candida glabrata ,candida parapsilosis ,candida krusei ,mucous spore bacterium (MSB) etc .Methods 24 PA isolates were collected from clinical specimens and identified by Gram′s stain ,oxidase production and the API 20NE system(bi-oMerieux ,France) .Cross-streaking method and sterilizing filter paper-disk method and co-cultured method were applied to observe the inhibitory effect of PA .Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analyzed the difference of bacte-rial proteins of PA .Results The results showed that some strains of 24 PA had strong inhibitory effect against pathogenic fungi , some strain had partial effect and others had no effect .Co-cultured test showed that PA could inhibit the growth of fungal hyphae . SDS-PAGE displayed the significant difference in secretive proteins between the PA strains which had strong effect and no effect . Conclusion PA have inhibitory effect upon common pathogenic fungi and and this might be related to inhibit fungal hyphae forma-tion ,various protein secretion and inhibit the growth of fungi .关键词
假单胞菌 ,铜绿/真菌/绿脓菌素/十字交叉划线法Key words
pseudomonas aeruginosa/fungi/pyocyanine/cross-streaking method引用本文复制引用
徐令清,汪峰,侯红艳,刘彩林,欧国平,孙明月,孙自镛..铜绿假单胞菌对致病真菌的抑制作用[J].重庆医学,2014,(7):769-771,778,4.基金项目
国家科技重大专项“十二五”资助课题（2012ZX10004207-004）。 （）
