重庆医学Issue(9)：1098-1100,1103,4.DOI:10.3969/j.issn.1671-8348.2014.09.027
纳米 HA／PVA 多孔复合水凝胶人工角膜支架材料对兔角膜成纤维细胞生长的影响
The effect on rabbit corneal fibroblasts cultivated on n-HA/PVA hydrogel composite
摘要
Abstract
Objective To evaluate the biocompatibility of nano-hydroxyapatite/polyvinyl alcohol (n-HA/PVA ) hydrogel com-posite for keratoprosthesis .Methods There were four materials to be evaluated ,big-porous and small-porous n-HA/PVA hydrogel composite ,big-porous and small-porous PVA hydrogel .Every kind of materials was digested in F12 nutrient culture for 24 h to get the materials impregnant .Corneal fibroblasts were cultivated in materials impregnant and through methyl thiazolyl tetrazolium col-orimetry to assay the cytotoxicity .Rabbit corneal fibroblasts were seeded onto the four materials in vitro .Three days later the growth of fibroblasts were observed by inverted microscope and scanning electron microscope (SEM ) .Results The cytotoxicity scores of the materials with big-porous were both grade one .The mean corneal fibroblasts counts on culture were no obvious differ-ence between big-porous materials groups and control group under plate inverted microscope 72 hours later(P=0 .608) .Through scanning electron microscope 72 hours later ,corneal fibroblasts grew well on n-HA/PVA hydrogel composite ,and migrated into the porous of composite materials .The corneal fibroblasts on big-porous n-HA/PVA hydrogel composite were more obviously found than the other three materials(F=95 .245 ,P=0 .000) .Conclusion The porous n-HA/PVA hydrogel composite is a kind of good biocompatible material which has no cytotoxicity .The n-HA/PVA hydrogel composite with big porous would be an ideal pe-ripheral skirt of keratoprosthesis .关键词
水凝胶/角膜/生物相容性材料/细胞毒性Key words
hydrogel composite/nanostructures/biocompatible materials/cytotoxicity引用本文复制引用
熊洁,邓应平,王语诗..纳米 HA／PVA 多孔复合水凝胶人工角膜支架材料对兔角膜成纤维细胞生长的影响[J].重庆医学,2014,(9):1098-1100,1103,4.
