动物医学进展Issue(11)：16-19,4.
维氏气单胞菌双基因PCR检测方法的建立
Establishment of Duplex PCR for Detecting Aeromonas veronii
摘要
Abstract
The duplex PCR was developed with genome DNA of Aeromonas veronii ATCC35624 as template and the primers designed according to 16 S rRNA and nuclease gene(exu gene) .The sensitivity and speci-ficity were verified and meanwhile the simulation polluted samples were detected by this method .The re-sults showed that the DNA fragments of approximate 880 bp and 320 bp were obtained .Both 16 S rRNA gene and exu gene shared 99% homology with these genes from Aeromonas veronii ATCC35624 .This method had high sensitivity with the detected DNA concentration approximate 1 .58 × 10-4 ng/μL and high specificity with only the standard strains been detected . The artifical simulation polluted experiments showed that the detected rate of this method with 90% was higher than it of bacterial cultivation with 73 .3% .The developed duplex PCR can overcome the shortage of traditional bacterial cultivation and pro-vide the new method to detect A eromonas veroni .关键词
维氏气单胞菌/16 SrRNA/核酸酶基因/聚合酶链反应Key words
Aeromonas veronii/16 S rRNA/nuclease gene/PCR引用本文复制引用
王惠,边宇,孟庆峰,杨滨僮,康元环,荆琦,单晓枫,钱爱东..维氏气单胞菌双基因PCR检测方法的建立[J].动物医学进展,2013,(11):16-19,4.基金项目
国家自然科学基金项目（31201927）；国家质检总局科技计划项目 （）
