动物医学进展Issue(11)：46-50,5.
抵抗素成熟肽的制备及其对猪葡萄糖和脂肪代谢的影响
Preparation of Resistin Mature Peptide and Its Effect on Glucose and Fat Metabolism in Pigs
摘要
Abstract
The present work was aimed to further investigate the effects of resistin mature peptide on glu-cose and fat metabolism in pigs .The fragment encoding for resistin mature peptide(MR) was cloned into the expression vector pET-32a(+ ) ,and expressed in Ecoli BL-21(DE3) .The recombinant MR fusion pro-tein was purified by Ni-NTA affinity chromatography .Then the fusion peptide was cleaved from the re-combinant MR fusion protein by enterokinase digestion ,and the purified MR was isolated by Ni-NTA af-finity chromatography subsequently .18 5-month-old castrated Bama pigs were randomly divided into 3 groups ,the pigs in control group received nomal saline 0 .5 mL intramuscularly ,and the animals in experi-mental groups respectively received 20 and 40 ng MR per kilogram body weight ,blood samples were col-lected at 0 ,1 ,2 ,3 ,4 ,5 h after administration ,and the biochemical parameters associated glucose and fat metabolism were detected .The results showed that the recombinant MR fusion protein with molecular weight of 29 ku ,MR cleaved fusion protein with molecular weight of 12 ku were detected by SDS-PAGE , and the concentration of purified MR was 3 mg/L .The results also revealed that the serum glucose levels of the pigs in the experimental groups were significantly increased (P<0 .05);the serum lipoprotein lipase activities were slightly elevated ,but no significant differences among control and experimental groups were observed(P>0 .05);the serum triglyceride contents of the animals in the experimental groups were signif-icantly decreased ,moreover ,the serum triglyceride level of the pigs in experimental group 2 was signifi-cantly lower than that of control and experimental group 1(P<0 .05);and the free fatty acid content of test group 2 was also significantly higher than that of control and test group 1(P<0 .05) .The results a-bove suggested that the glucose intake of porcine histocytes was inhibited and the triglycerides decomposi-tion was promoted along with increased serum lipoprotein lipase activity owing to high dose of MR admin-istration ,resistin may play an important role in the regulation of energy metabolism and homeostasis of an-imals .关键词
抵抗素成熟肽/葡萄糖代谢/脂肪代谢/猪Key words
resistin mature peptide/glucose metabolism/fat metabolism/pig分类
农业科技引用本文复制引用
白翠玲,罗丹,刘艳芬,陈绍红,刘铀..抵抗素成熟肽的制备及其对猪葡萄糖和脂肪代谢的影响[J].动物医学进展,2013,(11):46-50,5.基金项目
广东海洋大学人才引进项目 （）
