摘要
Abstract
A Newcastle disease virus (NDV ) was isolated from ND suspected chickens of a scale chicken farm in Shaanxi Province .The F gene of the Newcastle disease virus (NDV) Shaanxi isolate was amplified and genetic evolution analyzed .The results showed that the whole nucleotide sequence length of F gene was 1662bp .The sequence of amino acid at the cleavage site of F gene is 112R-R-Q-K-R-F117 ,which is consistent with the characteristics of strong NDV strain .Comparison of the NDV isolate with the strong NDV isolates of gene VII of epidemic strains from different countries showed that the nucleotide homology was 92 .4%-98 .0% ,and in which the comparison with the VIId were above 97 .3% .Comparison of the NDV isolate with the current vaccine strains such as Lasota (DQl95265 ) ,F48E9 (AY508514 ) ,B1 (AF309418) ,V4(AF217084) ,Mukteswar (EF201805) and so on showed that the nucleotide homology was 83 .2%-86 .0% . The phylogenetic tree analysis demonstrated that the NDV isolate and VIId strains of FJ882014-LN and FJ608335-XJ were located in the same cladogram branch .关键词
新城疫病毒/分离鉴定/F基因/序列分析/遗传进化/鸡Key words
Newcastle disease virus/isolation and identification/F gene/sequence analysis/genetic evolu-tion/chicken分类
农业科技
