动物医学进展Issue(11)：81-84,4.
普氏野马物种的PCR鉴定
PCR Identification for Equus przewalsrii Species
摘要
Abstract
To develop the species-specific PCR assay for the identification of Equus przewalsrii ,primers were designed from mitochondria D-loop region after alignment of the available sequences in the GenBank database .The primers generated specific fragments of 315 bp length for Equus przewalsrii .The results showed that the PCR was established .The sensitivity analysis showed that the developed PCR could detect 10-3 ng DNA ,and no cross reaction with Mongolia horse ,Ili horse ,Kazakh horse ,Arabian horse ,Don-key ,Mule ,Bovine and Ovine .The results demonstrated that this species-specific PCR provided a useful , specific ,sensitive and simple technique to identify Equus przewalsrii .This assay could play an important role in conservation of Equus p rz ew alsrii .关键词
普氏野马/物种鉴定/线粒体DNA/D-Loop区Key words
Equus przewalsrii/species identification/mitochondrial DNA (mtDNA)/D-loop region分类
农业科技引用本文复制引用
郑小龙,王群,岳志芹,朱来华,梁成珠,邓明俊,肖西志,姜帆,于业锋..普氏野马物种的PCR鉴定[J].动物医学进展,2013,(11):81-84,4.基金项目
国家科技支撑计划项目 （）
