动物医学进展Issue(12)：6-10,5.
鸡传染性法氏囊病病毒 RT-PCR 检测方法的建立与应用
Development and Application of RT-PCR for Detecting Avian Infectious Bursal Disease Virus
摘要
Abstract
Infectious bursal disease virus (IBDV)is one of three severe acute diseases in China.IBDV had emerged worldwide and caused great loss to poultry production.And IBD is a quarantine disease in the avian and avian product trade.The rapid diagnosis is crucial to any control program of IBD.In order to construct a protocol of mo-lecular diagnostic detection technique(MDD)for IBDV,two conventional RT-PCR assays directed at the VP2 gene in fragment A with 604bp production and VP1 gene of in fragment B with 642 bp respectively were optimized with the universal primers and IBDV-specific 3′extremity primers respectively.A Taqman real-time reverse transcrip-tion-PCR test IBDV(IBDV-VP2-rRT-PCR)directed at the VP2 gene in fragment A was developed specifically with 5 strains.The sensitivities of three kinds of MDD assays were evaluated.The sensitivities of IBDV-VP2(604)-RT-PCR and IBDV-VP1(642)-RT-PCR were equal in 10-3 dilution of IBDV-BLEN vaccine,and the sensitivity of IB-DV-VP2-rRT-PCR was ten times superior to two conventional RT-PCR assays in 10-4 dilution .The specificity of three MDD assays developed had no cross reaction with others.These methods potentially allowed for more rapid, sensitive,and specific detection with 221 field samples for five years with monitoring and surveillance of IBDV.关键词
传染性法氏囊病/常规 RT-PCR/实时荧光定量 RT-PCR/快速检测Key words
IBDV/RT-PCR/rRT-PCR/rapid detection分类
农业科技引用本文复制引用
孙洁,阮周曦,陶虹,张彩虹,刘建利,花群义,吕建强,秦智锋,卢体康,曾少灵,杨俊兴,詹爱军,林庆燕,曹琛福,陈兵,廖立珊..鸡传染性法氏囊病病毒 RT-PCR 检测方法的建立与应用[J].动物医学进展,2013,(12):6-10,5.基金项目
国家标准制定基金项目（20072030-T-326）；深圳检验检疫局科研课题 （）
