动物医学进展Issue(12)：29-33,5.
副猪嗜血杆菌和猪传染性胸膜肺炎放线杆菌双重 PCR 检测方法的建立及应用
Establishment and Application of Duplex PCR for Detection of Haemophilus parasuis and Actinobacillus pleuropneumoniae
摘要
Abstract
The duplex PCR was developed for detecting Haemophilus parasuis (Hps)and Actinobacillus pleuropneumoniae (App).The 821 bp and 346 bp DNA fragments were specifically amplified,respective-ly,which were based on 16 S rRNA of Hps gene and App ApxIVA.The assay could not amplify any genes of Bordetella bronchiseptica ,Pasteurella multocida ,Escherichia coli ,Salmonella,Staphylococcus au-reus ,PCV-2,SIV and PRRSV.The duplex PCR could detect 38 pg/mL of Hps,3.8 pg/mL of App for DNA,as well as 8.9 cfu of Hps and 26.9 cfu of App for bacteria.The duplex PCR and bacterial culture were used to analyze lung samples from slaughter house pigs.The duplex PCR was positive in 18 of 36 clinical samples and Hps was isolated from 13 of 36 of these samples,which were consistent with the sin-gle PCR method to detect Hps and App.Furthermore,all of reference and clinical strains and artificial samples could be identified by this method.Therefore,the results showed that the duplex PCR is suitable for the detection of Hps and App in clinic.关键词
副猪嗜血杆菌/猪传染性胸膜放线杆菌/双重PCR方法Key words
Haemophilus parasuis/Actinobacillus pleuropneumoniae/duplex PCR分类
农业科技引用本文复制引用
余远迪,曾泽,岳华,张斌..副猪嗜血杆菌和猪传染性胸膜肺炎放线杆菌双重 PCR 检测方法的建立及应用[J].动物医学进展,2013,(12):29-33,5.基金项目
“十二五”国家高技术研究发展（863）计划项目（2012AA101304）；西南民族大学中央高校基本科研项目 （）
