动物医学进展Issue(12)：34-37,4.
禽白血病病毒 p27蛋白单克隆抗体的制备及鉴定
Preparation and Characterization of the Monoclonal Antibodies Against p27 Protein of Avian Leukosis Virus
摘要
Abstract
The purified recombinant protein p27 of avian leukosis virus as an immunogen was used to immu-niz Balb/c mice.By cell culture and monoclonal antibody of dilution method,mAb named 2F3,5C2 and 5C7 were prepared against avian leukosis virus p27 protein by fusing myeloma cell line SP2/0 with spleen lymphocytes of immunized Balb/c mice.By biological characteristic analysis,the number of three strain hybridoma cell chromosomes was about 90.Then immunological identifications were conducted by means of ELISA/Western blot and IFA,the three mAb against p27 protein of ALV possessed high antibody ti-ters and good specificity.The result showed that the three McAbs had good reactogenicity.These three McAbs can be used as a valuable tool for avian leukosis virus epitope analysis,the detection of the virus and Kit research.关键词
禽白血病病毒/p27 蛋白/单克隆抗体Key words
ALV/p27 protein/monoclonal antibody分类
农业科技引用本文复制引用
谢华丽,邵华斌,杨峻,程国富,胡薛英,谷长勤,张万坡,罗青平..禽白血病病毒 p27蛋白单克隆抗体的制备及鉴定[J].动物医学进展,2013,(12):34-37,4.基金项目
现代农业产业技术体系建设专项（nycytx-42-G3-04）；湖北省重点实验室项目（2010ZD108）；湖北省畜禽流感等主要疫病防控技术集成与示范研究开发项目 （）
