动物医学进展Issue(12)：42-45,4.
SIV、PCV-2、PRRSV 三重 PCR 检测方法的建立
Establishment and Application of a triplex PCR for Detection of SIV,PCV-2 and PRRSV
摘要
Abstract
According to the sequences reported in GenBank of virus conserved sequences,three pairs of primer were synthesized to amplify target sequences of SIV、PCV-2、PRRSV.Through the optimization of reaction conditions and the detection of clinical cases,the SIV,PCV-2 and PRRSV triplex PCR was established.The results show that the triplex PCR had the minimum detection limit10-4-10-5 .40 clinical samples were detected by triplex PCR and single PCR/RT-PCR,the consistent rate was 100%.The results suggest that:The triplex PCR can be used for the detection of clinical samples with single or coinfections with three kinds of viruses.关键词
猪流感病毒/猪圆环病毒2型/猪繁殖与呼吸综合征病毒/三重PCRKey words
Swine influenza virus/Porcine circovirus type 2/Porcine reproductive and respiratory syndrome virus/triplex PCR分类
农业科技引用本文复制引用
陈胜锋,徐振娜,王丙云,计慧琴,陈志胜..SIV、PCV-2、PRRSV 三重 PCR 检测方法的建立[J].动物医学进展,2013,(12):42-45,4.基金项目
佛山市科技专项发展基金项目（2009030）；广东省佛山市禅城区科技开发专项资金项目 （）
