动物医学进展Issue(12)：115-119,120,6.
柔嫩艾美耳球虫长春株 SAG2基因的克隆与序列分析
Cloning and Sequence Analysis of SAG2 Gene in Eimeria tenella Changchun Strain
摘要
Abstract
According to the SAG2 gene sequence of E.tenella published in GenBank,a pair of specific prim-ers was designed.Then,the SAG2 gene in E.tenella Changchun strain was amplified by RT-PCR and li-gated into pMD18T vector,the positive clone was sequenced.The results showed that the ORF of E. tenella of Changchun strain SAG2 gene is 813 bp,compared with the published SAG2 gene (Houghton strain)sequence,four nucleotide mutation sites appeared in the SAG2 gene of E.tenella Changchun strain.There is 99.50% homology in nucleotide sequence,and 99.63% homology in its encoding region sequence.Among these mutation sites,A185G mutation led to substitution of glutamic acid by glycine, and A247T mutation led to substitution of methionine by leucine.There is strong hydrophobic at N-termi-nal and C-terminal of SAG2,and there is 23 amino acid transmembrane signal peptide at N-terminal,how-ever,there are a lot of hydrophilic zones in the middle portion,and showed strong antigenicity.These suggest that there is high homology between Changchun strain and Houghton strain of E.tenella SAG2 gene,the protein encoded by SAG2 gene in E.tenella Changchun strain had strong antigenicity,it can be used as a candidate antigen for vaccine development.关键词
柔嫩艾美耳球虫长春株/SAG2 基因/克隆/序列分析Key words
Eimeria tenella Changchun strain/SAG2 gene/cloning/sequence analysis分类
农业科技引用本文复制引用
曹利利,姚新华,侯洪烈,苑淑贤,任科研..柔嫩艾美耳球虫长春株 SAG2基因的克隆与序列分析[J].动物医学进展,2013,(12):115-119,120,6.基金项目
吉林省科技发展计划项目（201205071）；辽宁省丹东市科学技术计划项目 （）
