动物医学进展Issue(12):213-217,5.
银环蛇奇异变形杆菌的分离鉴定与药敏试验
Isolation,Identification and Drug Sensitivity Test of Proteus mirabilis from Bungarus multicinctus
摘要
Abstract
Coral snake (Bungarus multicinctus )was used as one of valued traditional Chinese medicine.If mismanagement existed during breeding,the snakes were easily infected with bacteria,thus hindering the development of the artificial culturing coral snake.Herein,we dissected diseased coral snake specimens, then isolated and purified bacterial strains.16 S rDNA for bacterial strains were sequenced and compared with that of other bacterial strains recorded in GenBank,and finally phylogenetic tree was constructed. Our results suggested that the two bacterial strains belonged to Proteus.One of them was Proteus mirabi-lis .For the first time,we identified P .mirabilis from heart and kidney of coral snakes.Drug sensitivity test indicated that the two strains were highly sensitivity to ceftriaxone sodium,cefepime and amikacin, and were medium or low sensitivity to amoxicillin,streptomycin,but were resistant to ciprofloxacin,nor-floxacin,and ampicillin.The results have a certain value for clinical applications for breeding coral snakes.关键词
银环蛇/奇异变形杆菌/药敏试验Key words
Bungarus multicinctus/Proteus mirabilis/drug sensitivty test分类
农业科技引用本文复制引用
赵成坚,黄勇,谷颖乐,徐永莉,张月云,李力,曾芸..银环蛇奇异变形杆菌的分离鉴定与药敏试验[J].动物医学进展,2013,(12):213-217,5.基金项目
广西科技攻关与新产品试制项目(桂科攻10183010);广西科学研究与技术开发计划项目(12118005-2-5);南宁市科学研究与技术开发项目(20123238);广西卫生厅 ()