动物医学进展Issue(3)：15-18,4.
猪繁殖与呼吸综合征病毒TJM株Nsp2基因缺失片段的原核表达及免疫活性分析
Prokaryotic Expression and Immunogenicity Analysis of Deleted Region of PRRSV TJM Strain Nsp2 Gene
摘要
Abstract
An attenuated porcine reproductive and respiratory syndrome virus (PRRSV)TJM strain with Nsp2 deletions derived from a highly pathogenic PRRSV TJ strain by cloning and purificationon Marc-145 cells.The specific primers were synthesized according to the sequence of porcine reproductive and respirato-ry syndrome virus TJ strain F3 published in GenBank.The deleted region of PRRSV TJM strain Nsp2 was amplified by RT-PCR and cloned into pGEX-6p-1 to construct the recombinant plasmid GST-dNsp2.The recombinant vector was transformed into E.coli BL21(DE3)and expressed.After purification with affinity column,GST-dNsp2 protein was purified and concentration can be up to 1 .6 mg/mL.The targeted protein dNsp2 was dissociated from fusion protein GST-dNsp2 by the PreScissionTM protease,concentration was 0.41 mg/mL.The results showed GST-dNsp2 and dNsp2 had good immunologic activities.The high level expression of dNsp2 laid a basis for establishment of an ELISA for distinguishing PRRSV TJ strain from TJM strain.关键词
猪繁殖与呼吸综合征病毒/Nsp2 基因/原核表达Key words
Porcine reproductive and respiratory syndrome virus/Nsp2 gene/prokaryotic expression分类
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黄双,王凤雪,温永俊,高维凡..猪繁殖与呼吸综合征病毒TJM株Nsp2基因缺失片段的原核表达及免疫活性分析[J].动物医学进展,2014,(3):15-18,4.基金项目
国家863计划项目（2011AA10A213）；吉林省特种经济动物疫病防控研究创新团队项目 （）
