动物医学进展Issue(3)：92-96,97,6.
西尼罗病毒病诊断技术研究进展
Progress on Diagnostic Techniques of West Nile Virus Disease
摘要
Abstract
West Nile virus disease (WND)is a seriously infectious zoonosis caused by West Nile virus (WNV).The application of different diagnostic tests for accurate and timely diagnosis of the disease is the premise for the prevention and control of WND.This article mainly reviewed the latest research progress on diagnostic techniques for WND.The diagnostic methods of WND include pathogenical,serological and molecular diagnoses.In the pathogenical detection aspect,these techniques include virus isolation and iden-tification,antigen capture ELISA and membrane-based electrochemical nanobiosensor.In the serological detection aspect,mainly include ELISA,complement-dependent cytotoxicity (CDC),microsphere-based im-munoassay (MIA)and surface enhanced Raman scattering immunoassay(SERS).In molecular detection aspect,mainly include RT-PCR,RT-nPCR,real-time RT-PCR,loop-mediated isothermal amplification as-say (LAMP)and nucleic acid sequence-based amplification assay (NASBA).In addition,the advantages, disadvantages and prospects of the several methods were also discussed,to provide some references for ap-plication and future research.关键词
西尼罗病毒病/诊断技术/病原学/血清学/分子生物学Key words
West Nile virus disease/diagnostic technique/pathogen/serology/molecular biology分类
农业科技引用本文复制引用
郑小龙,朱来华,王群,孙涛,孙明君,邓明俊,张晓文..西尼罗病毒病诊断技术研究进展[J].动物医学进展,2014,(3):92-96,97,6.基金项目
国家质检总局科技计划项目 （）
